![]() For example, you need to do such dilutions of the standard IgG to make the standard curve in ELISA, and then again for the unknown samples in ELISA. Often in experimental work, you need to cover a range of concentrations, so you need to make a bunch of different dilutions. Once you understand these principles, you will be better able to design the dilutions you need for each specific case. Advantages And Disadvantages Of Serial Dilution-agar Plate It explains some principles for designing dilutions that give optimal results. There is problem of.Īdvantages of 'Serial Dilutions' This section is not a recipe for your experiment. The temperature of the medium need to be carefully controlled: too hot will kill the microorganisms, too cold will create clumps of congealed agar, which can sometimes be mistaken for colonies. Disadvantages: It is time consuming especially when diluting the original sample by serial dilution. INTRODUCTION In order to be able to adequately study and characterize a. THE ADVANTAGES AND DISADVANTAGES OF THE VARIOUS MICROBIAL CULTURE TECHNIQUES.
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